SCIENTIFIC PROGRAMME COMMITTEE

______________________________________________________________

SUNDAY – 03 October 2004

______________________________________________________________

15:00 Registration Desk Open

Mounting of Posters

16:00 – 17:00 Conference buses from Bratislava and Vienna arrive at Smolenice Castle

18:00 – 19:15 Chairman: Stefan Janecek

Keynote Lecture - L1

Pedro M. Coutinho (Marseille, France): Starch-active enzymes and related proteins: data integration for evolutionary and functional analysis.

19:30 – 22:00 Opening Reception

______________________________________________________________

MONDAY – 04 October 2004

______________________________________________________________

07:30 – 08:30 Breakfast

08:25 Announcements

08:30 – 12:10 Chairmen: Torben V. Borchert and Birte Svensson

Clan GH-H: Structure-function relationships I.

08:30 – 09:15 L2

Nushin Aghajari (Lyon, France): Carbohydrate recognition, binding and processing in barley alpha-amylase isozymes.

09:15 – 09:40 L3

Stephanie Ravaud (Lyon, France): Crystallographic study of the sucrose isomerase MutB from Pseudomonas mesoacidophila.

09:40 – 10:05 L4

Thomas R.M. Barends (Groningen, The Netherlands): The crystal structure of Aquifex aeolicus amylomaltase explains the ring size of its cycloamylose product.

10:05 – 10:35 Coffee/Tea Break and Posters

10:35 – 11:00 L5

Kazutoshii Fujii (Osaka, Japan): Protein engineering of amylomaltase from Thermus aquaticus with random and saturation mutageneses.

11:00 – 11:25 L6

Slavko Kralj (Haren, The Netherlands): Molecular and biochemical characterization of glucansucrases from lactobacilli.

11:25 – 12:10 L7

Narayanan Ramasubbu (Newark, NJ, USA): Structure-function relationships in human salivary alpha-amylase: role of aromatic residues.

12:10 – 13:30 Lunch

13:30 – 15:00 Poster Session 1

15:00 – 18:40 Chairmen: Richard Haser and Pierre Monsan

Clan GH-H: Structure-function relationships II.

15:00 – 15:45 L8

Lubbert Dijkhuizen (Haren, The Netherlands): Structure/function relationships determining product and reaction specificities in cyclodextrin glycosyltransferase.

15:45 – 16:10 L9

Hossein Naderi-Manesh (Tehran, Iran): Structural investigation and homology modeling studies of a Ca-independent alpha-amylase purified from Bacillus sp. KR-8104: active and stable at low pH.

16:10 – 16:35 L10

Ge-xin Zhang (Jiangsu, China): The alpha-amylase amino acid feature in different kingdoms.

16:35 – 17:05 Coffee/Tea Break and Posters

17:05 – 17:30 L11

Shukun Yu (Copenhagen, Denmark): The anhydrofructose pathway of glycogen and starch degradation.

17:30 – 17:55 L12

Romina Rodriguez-Sanoja (Mexico, Mexico): Functional characteristics of the Lactobacillus amylovorus alpha-amylase starch binding domain.

17:55 – 18:40 L13

Birte Svensson (Kgs. Lyngby, Denmark): Binding of carbohydrates and protein inhibitors at the surface of alpha-amylases.

18:40 – 20:00 Supper

______________________________________________________________

TUESDAY – 05 October 2004

______________________________________________________________

07:30 – 08:30 Breakfast

08:25 Announcements

08:30 – 12:10 Chairmen: Bauke W. Dijkstra and E. Ann MacGregor

“Cousin” families.

08:30 – 09:15 L14

Atsuo Kimura (Sapporo, Japan): Molecular analysis of alpha-glucosidase (GH-family 31).

09:15 – 09:45 L15

Masahiro Mizuno (Tokyo, Japan): Three-dimensional structure of glucodextranase, glycoside hydrolase family 15 enzyme.

09:45 – 10:15 L16

Jozef Sevcik (Bratislava, Slovakia): Structure of the complex of S. fibuligera glucoamylase with acarbose indicates the presence of the starch-binding site in the catalytic domain.

10:15 – 10:45 Coffee/Tea Break and Posters

10:45 – 11:15 L17

Heidi A. Ernst (Copenhagen, Denmark): Probing the structure of glucan lyases - the lytic members of GH-31 - by sequence analysis, circular dichroism and proteolysis.

11:15 – 12:00 L18

Michael J. O’Donohue (Reims, France): Characterization of a GH57 amylopullulanase: a survey of functionality and active site organisation.

12:00 – 13:30 Lunch

14:00 Trip by boat to water dam Gabcikovo (conference buses leave for Bratislava)

19:00 – 21:00 Dinner

24:00 Arrival at the Smolenice Castle

______________________________________________________________

WEDNESDAY – 06 October 2004

______________________________________________________________

07:30 – 08:30 Breakfast

08:25 Announcements

08:30 – 12:10 Chairmen: Takashi Kuriki and John F. Robyt

Clan GH-H: Protein Engineering and Laboratory Evolution.

08:30 – 09:15 L19

Bart A. van der Veen (Toulouse, France): CEASE: Combinatorial Engineering to enhance Amylosucrase performance.

09:15 – 09:40 L20

Kwan-Hwa Park (Seoul, Korea): Dissociation/association properties of cyclodextrin-hydrolyzing enzyme: an oligomeric state analysis by pH and salt.

09:40 – 10:05 L21

Sophie Bozonnet (Kgs. Lyngby, Denmark): Molecular evolution techniques as tools to improve barley alpha-amylase AMY2 expression.

10:05 – 10:35 Coffee/Tea Break and Posters

10:35 – 11:00 L22

Thijs Kaper (Groningen, The Netherlands): Engineering the reaction specificity of GH77 amylomaltase from Thermus thermophilus HB8 by laboratory evolution.

11:00 – 11:25 L23

Lars Beier (Bagsvaerd, Denmark): Specificity engineering of a maltogenic alpha-amylase.

11:25 – 12:10 L24

Lars Skov (Copenhagen, Denmark): Structural studies of sucrose utilizing enzymes from glycoside hydrolase family 13.

12:10 – 13:30 Lunch

13:00 – 17:00 Trip to Cave “Driny” (optional; otherwise free time)

17:00 – 17:45 Chairman: Stefan Janecek

Special Lecture - L25

Hannes Lohi (Toronto, Canada): Laforin preferentially binds the neurotoxic starch-like polyglucosans, which form in its absence in progressive myoclonus epilepsy.

17:45 – 19:15 Poster Session 2 (including Refreshment)

19:15 – 20:00 Chairman: Birte Svensson

Banquet Lecture - L26

E. Ann MacGregor (West Lothian, U.K.): An overview of clan GH-H and distantly-related families.

20:00 – 24:00 Symposium Banquet (+ Poster Prizes)

______________________________________________________________

______________________________________________________________

THURSDAY – 7 October 2004

______________________________________________________________

07:00 – 08:00 Breakfast

Vacation of the rooms; Poster removing

08:00 – 08:10 Announcements

08:10 – 11:55 Chairmen: Marc van der Maarel and Kwan-Hwa Park

Family GH-13 Enzymes and Inhibitors.

08:10 – 08:55 L27

Bharat Patel (Brisbane, Queensland, Australia): The thermohalophilic amylases of Halothermothrix orenii.

08:55 – 09:20 L28

Lili Kandra (Debrecen, Hungary): Transglycosylations catalysed by alpha-amylases.

09:20 – 09:45 L29

Pernilla Turner (Lund, Sweden): Two novel cyclodextrin-degrading enzymes isolated from thermophilic bacteria, have similar domain structures but differ in oligomeric state and activity profiles.

09:45 – 10:05 Coffee/Tea Break

10:05 – 10:30 L30

Elspeth A. MacRae (Auckland, New Zealand): Characterisation of putative alpha-amylases from apple (Malus domestica) and Arabidopsis thaliana.

10:30 – 11:15 L31

John F. Robyt (Ames, IA, USA): Inhibition, activation and stabilization of alpha-amylases.

11:15 – 11:55 Closing Lecture - L32

Takashi Kuriki (Osaka, Japan): The conclusive proof that supports the concept of the alpha-amylase family: structural similarity and common catalytic mechanism.

11:55 – 12:00 Closure of ALAMY_2

12:00 The 1^st Conference Bus leaves for Bratislava and Vienna

12:00 – 13:00 Lunch

13:00 The 2^nd Conference Bus leaves for Bratislava and Vienna

______________________________________________________________